| 184 | 0 | 52 |
| 下载次数 | 被引频次 | 阅读次数 |
稻米的外观品质对稻米市场价格有着重要影响。通过检测332份热带稻区水稻材料2 a间2个种植季中的精米粒长、粒宽、长宽比、垩白度、垩白粒率和透明度等6个外观性状，并基于水稻40 K SNP液相芯片的基因型数据，采用Q+K混合模型开展全基因组关联分析（GWAS)。结果表明，共检测到17个与外观品质性状相关的QTL，其中7个QTL表现出明显的多效性，同时控制2或3个性状；11个QTL在2 a中被重复检测到，其中有6个QTL区域位于已克隆的粒型基因区域。研究结果为揭示稻米外观品质的遗传基础及后续基因挖掘与分子育种提供了重要参考。
Abstract：The appearance quality is a key determinant of the market value of rice. In this study, six appearance quality traits, including grain length, grain width, length-to-width ratio, chalkiness degree, chalky grain rate and translucency degree of milled rice, were measured for 332 tropical rice germplasm accessions across two growing seasons in two years. Based on the rice genotype data obtained by the 40 K SNP liquid-phase chip, a mixed linear model(Q+K) was employed for the genome-wide association study(GWAS). The results showed that a total of 17 quantitative trait loci(QTLs) associated with rice appearance quality were identified. Among them, seven QTLs showed pleiotropic effects on two or three traits. Eleven QTLs were consistently detected in both seasons, and six QTLs were located in or near the regions harboring previously cloned grain shape-related genes. These findings provide new insights into the genetic basis of rice appearance quality and offer valuable resources for gene mining and molecular breeding.
[1] BAI S W, YU H, WANG B, et al. Retrospective and perspective of rice breeding in China[J]. Journal of genetics and genomics,2018,45(11):603-612.
[2] SONG X G, MENG X B, GUO H Y, et al. Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size[J]. Nature biotechnology,2022,40(9):1403-1411.
[3] ZHAO D S, LI Q F, ZHANG C Q, et al. GS9 acts as a transcriptional activator to regulate rice grain shape and appearance quality[J]. Nature communications, 2018,9(1):1240.
[4]邱颖欣,董皓,李懿星.等.水稻垩白性状相关基因研究进展[J].杂交水稻, 2013,38(4):12-20.
[5]李然,钱前,高振宇.水稻品质的遗传与育种改良研究进展[J].生物技术通报, 2022,38(4):4-19.
[6] WU B, YUN P, ZHOU H, et al. Natural variation in WHITECORE RATE 1 regulates redox homeostasis in rice endosperm to affect grain quality[J]. The plant cell, 2022,34(5):1912-1932.
[7] YANO K, MORINAKA Y, WANG F M, et al. GWAS with principal component analysis identifies a gene comprehensively controlling rice architecture[J]. Proceedings of the National Academy of Sciences of the United States of America,2019,116(42):21262-21267.
[8]任生林,吴才文,经艳芬,等.全基因组关联分析在作物中的研究进展[J].分子植物育种, 2024,22(11):3594-3602.
[9] PRITCHARD J K, STEPHENS M, DONNELLY P. Inference of population structure using multilocus genotype data[J]. Genetics,2000,155(2):945-959.
[10] LIPKA A E, TIAN F, WANG Q S, et al. GAPIT:Genome association and prediction integrated tool[J]. Bioinformatics,2012,28(18):2397-2399.
[11] RUAN B P, SHANG L G, ZHANG B, et al. Natural variation in the promoter of TGW2 determines grain width and weight in rice[J]. New phytologist, 2020,227(2):629-640.
[12] FAN C C, XING Y Z, MAO H L, et al. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein[J].Theoretical and applied genetics, 2006,112(6):1164-1171.
[13] LI Q P, LU L, LIU H Y, et al. A minor QTL, SG3, encoding an R2R3-MYB protein, negatively controls grain length in rice[J].Theoretical and applied genetics, 2020,133(8):2387-2399.
[14] ZHANG X J, WANG J F, HUANG J, et al. Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(52):21534-21539.
[15] LI Y B, FAN C C, XING Y Z, et al. Natural variation in GS5plays an important role in regulating grain size and yield in rice[J]. Nature genetics, 2011,43(12):1266-1269.
[16] LIU J F, CHEN J, ZHENG X M, et al. GW5 acts in the brassinosteroid signalling pathway to regulate grain width and weight in rice[J]. Nature plants, 2017,3:17043.
[17] WANG Y X, XIONG G S, HU J, et al. Copy number variation at the GL7 locus contributes to grain size diversity in rice[J]. Nature genetics, 2015,47(8):944-948.
[18] WANG S K, WU K, YUAN Q B, et al. Control of grain size,shape and quality by OsSPL16 in rice[J]. Nature genetics, 2012,44(8):950-954.
[19] HUANG X H, WEI X H, SANG T, et al. Genome-wide association studies of 14 agronomic traits in rice landraces[J].Nature genetics, 2010,42(11):961-967.
[20] FENG Y, LU Q, ZHAI R R, et al. Genome wide association mapping for grain shape traits in indica rice[J]. Planta,2016,244(4):819-830.
[21] ZHENG X M, GONG T T, OU H L, et al. Genome-wide association study of rice grain width variation[J]. Genome,2018,61(4):233-240.
[22] MA X S, FENG F J, ZHANG Y, et al. A novel rice grain size gene OsSNB was identified by genome-wide association study in natural population[J]. PLoS genetics, 2019,15(5):e1008191.
[23]龚成云,朱裕敬,桂金鑫,等.水稻粒型全基因组关联分析[J].农业生物技术学报, 2024,32(11):2447-2461.
基本信息:
DOI：10.16267/j.cnki.1005-3956.20241123.255
中图分类号:S511
引用信息:
[1]刘法谋,黄智,郭梁等.全基因组关联分析热带稻区水稻外观品质相关性状的QTL[J].杂交水稻,2025,40(04):26-32.DOI:10.16267/j.cnki.1005-3956.20241123.255.
基金信息:
援埃塞俄比亚农业职业教育第三期技术援助项目[（2021）商合促技字第37号]